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KMID : 0364820140500020095
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Korean Journal of Microbiology
2014 Volume.50 No. 2 p.95 ~ p.100
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Characterization of Hrq1-Rad14 Interaction in Saccharomyces cerevisiae
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Min Moon-Hee
Kim Min-Ji
Choi You-Jin
You Min-Ju
Kim Uy-Ra
An Hyo-Bin
Kim Chae-Hyun
Kwon Chae-Yeon
Bae Sung-Ho
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Abstract
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Hrq1 is a novel member of RecQ helicase family, found in fungal genomes by bioinformatics analyses. It is most
homologous to human RECQL4 and recent genetic and biochemical studies suggested that it may play roles in the
maintenance of genome stability. In this study, we investigated yeast two-hybrid interactions between Hrq1 and the
yeast genes homologous to the human genes that are known to interact with RECQL4. Among the 11 genes tested,
Rad14, a nucleotide excision repair (NER) factor, was found to interact with Hrq1. In addition, pull-down assay with
the purified proteins revealed direct protein-protein interaction between Hrq1 and Rad14. The yeast two-hybrid
interaction was enhanced by the DNA damage induced by 4-nitroquinoline-1-oxide, which was dependent on the
presence of Rad4, a key NER factor. These results suggest that Hrq1 may function in NER through interaction with
Rad14.
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KEYWORD
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Hrq1, nucleotide excision repair, Rad4, Rad14, RecQ helicase, RECQL4 orthologoue
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